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Fig. 5 | Skeletal Muscle

Fig. 5

From: Whole-body clearing, staining and screening of calcium deposits in the mdx mouse model of Duchenne muscular dystrophy

Fig. 5

Comparative imaging of whole-body cleared mice. a Bright-field images (dorsal view) of perfusion-based CUBIC-cleared mdx model of DMD and control mouse stained with alizarin red S. Visible skeleton and calcifications within muscles (arrows). b Fluorescent image of mice with visible skeleton, calcifications within muscles and selected planes (1–10) in c magnified views. c Arrowheads indicate clusters of pathological calcium deposits in forelimb (1, 6), diaphragm (2, 7), lumbar region (3, 8), pelvic region (4, 9), and hindlimb (5, 10). Scale bar, 1 cm

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