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Fig. 1 | Skeletal Muscle

Fig. 1

From: A robust Pax7EGFP mouse that enables the visualization of dynamic behaviors of muscle stem cells

Fig. 1

EGFP expression in MuSCs of Pax7EGFP muscles. a Representative flow cytometry plots of MuSC populations (CD11b/CD31/CD45/Sca1/CD34+/α7-integrin+) derived from control (Pax7EGFP negative) and Pax7EGFP mice (left). MuSCs were then analyzed for the presence of GFP (right). b Assessment of the percent of MuSCs from (a) that are GFP+. c Analysis of GFP+ cells that are also CD34+ and α7-integrin+. d Immunofluorescence staining of FACS-isolated MuSCs for Pax7 and GFP show all cells co-express both markers. Scale bar = 100 μm. n > 3 mice per genotype

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