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Fig. 2 | Skeletal Muscle

Fig. 2

From: A robust Pax7EGFP mouse that enables the visualization of dynamic behaviors of muscle stem cells

Fig. 2

Myogenic potential of Pax7EGFP MuSCs in vitro. a MuSCs from either genotype were plated on laminin-coated chamber slides in growth medium for 2 days, prior to fixation and staining for Pax7 and MyoD. Scale bar = 100 μm. b Quantitation of MyoD positively stained cells in (a). n ≥ 3 mice per genotype and N > 500 cells per condition analyzed. c MuSCs from control or Pax7EGFP mice were grown first in proliferation medium for 5 days and then switched to differentiation medium for 2 days, fixed, and stained for the differentiation markers Myosin Heavy Chain (MyHC) and myogenin. Scale bar = 100 μm. d Quantitation of data displayed above. n ≥ 3 mice analyzed per group with N > 1000 cells analyzed per group

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