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Fig. 4 | Skeletal Muscle

Fig. 4

From: A robust Pax7EGFP mouse that enables the visualization of dynamic behaviors of muscle stem cells

Fig. 4

GFP expression patterns in tissues of Pax7EGFP mice. a Whole embryos were isolated from timed matings of Pax7EGFP heterozygous mice with wild-type mice and imaged for green fluorescence. Images were merged with brightfield images. Top: e11.5 dpc embryos. Note expression of GFP in the nasal region and somites. Middle: imaging of e14.5 dpc embryos. Bottom: close-up image of e14.5 dpc embryos demonstrating expression in the forelimb and hindlimb musculature. b Pax7 and GFP co-localize in vivo. Non-injured TA muscles were fixed and stained with antibodies against Pax7 and GFP, and co-stained with both DAPI and fluorescently tagged-WGA (ECM). Scale bar = 50 μm. Note the clear overlap in expression. Data were confirmed in n > 4 mice. c EGFP signal is present in TA tissue sections of Pax7EGFP mice without the need to stain with GFP antibody. d Two photon imaging of the tibialis anterior (longitudinal) of live Pax7EGFP mice in vivo. Left: muscle fibers. Middle: Pax7EGFP positive cells. Right: Merged image. Note the distinct GFP signal present in the satellite cell position. Scale bar = 200 μm

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