Fig. 5

Histological analysis of Pax7EGFP mice. a Representative images of gastrocnemius muscles from control (Pax7EGFP negative, top) or Pax7EGFP heterozygous mice (Bottom) stained with hematoxylin and eosin. b Quantitation of the gastrocnemius muscle weight normalized to body weight of each mouse. Further analyses included analysis of (c) fiber area of the gastrocnemius muscles between genotypes and (d) number of muscle fibers per section. n ≥ 3 age and gender-matched mice per genotype per condition. e Representative images of control and Pax7EGFP tibialis anterior muscles following injury. Tibialis anterior muscles were injured with notexin or left non-injured (left), and muscles were harvested 5 (middle) or 10 (right) days post-injury. Muscles were cryosectioned and stained with hematoxylin and eosin and imaged. Scale bar = 100 μm. f and g Quantitation of centrally nucleated fibers. n ≥ 3 mice per genotype per condition. N > 500 fibers analyzed per genotype per condition