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Table 2 Acquisition settings of tested images for the developed analysis feature

From: MuscleJ: a high-content analysis method to study skeletal muscle with a new Fiji tool

Acquisition Analysis
centro-nucleated fibers Vessels Satellite cells Fiber type
Image dimensions Z-Stack No No No No
Channels 2 3 3 4
Scaling xy (per pixel) 0.325 μm × 0.325 μm 0.325 μm × 0.325 μm 0.38 μm × 0.38 μm 0.325 μm × 0.325 μm
Acquisition information Microscope AxioScan.Z1 AxioScan.Z1 LSM 700 AxioScan.Z1
  Objective 20× 20× 40× 20×
Channel 1 Reflector DAPI DAPI DAPI DAPI
Excitation wavelength 353 353 404 353
Emission wavelength 465 465 444 465
Channel2 Reflector FITC Cy3 Cy3 Cy3
Excitation wavelength 495 548 561 548
Emission wavelength 519 561 575 561
Channel 3 Reflector   FITC FITC FITC
Excitation wavelength   495 488 495
Emission wavelength   519 517 519
Channel 4 Reflector     Cy5
Excitation wavelength     650
Emission wavelength     673