From: MuscleJ: a high-content analysis method to study skeletal muscle with a new Fiji tool
Acquisition | Analysis | ||||
---|---|---|---|---|---|
centro-nucleated fibers | Vessels | Satellite cells | Fiber type | ||
Image dimensions | Z-Stack | No | No | No | No |
Channels | 2 | 3 | 3 | 4 | |
Scaling xy (per pixel) | 0.325 μm × 0.325 μm | 0.325 μm × 0.325 μm | 0.38 μm × 0.38 μm | 0.325 μm × 0.325 μm | |
Acquisition information | Microscope | AxioScan.Z1 | AxioScan.Z1 | LSM 700 | AxioScan.Z1 |
Objective | 20× | 20× | 40× | 20× | |
Channel 1 | Reflector | DAPI | DAPI | DAPI | DAPI |
Excitation wavelength | 353 | 353 | 404 | 353 | |
Emission wavelength | 465 | 465 | 444 | 465 | |
Channel2 | Reflector | FITC | Cy3 | Cy3 | Cy3 |
Excitation wavelength | 495 | 548 | 561 | 548 | |
Emission wavelength | 519 | 561 | 575 | 561 | |
Channel 3 | Reflector | FITC | FITC | FITC | |
Excitation wavelength | 495 | 488 | 495 | ||
Emission wavelength | 519 | 517 | 519 | ||
Channel 4 | Reflector | Cy5 | |||
Excitation wavelength | 650 | ||||
Emission wavelength | 673 |