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Table 2 Acquisition settings of tested images for the developed analysis feature

From: MuscleJ: a high-content analysis method to study skeletal muscle with a new Fiji tool

Acquisition

Analysis

centro-nucleated fibers

Vessels

Satellite cells

Fiber type

Image dimensions

Z-Stack

No

No

No

No

Channels

2

3

3

4

Scaling xy (per pixel)

0.325 μm × 0.325 μm

0.325 μm × 0.325 μm

0.38 μm × 0.38 μm

0.325 μm × 0.325 μm

Acquisition information

Microscope

AxioScan.Z1

AxioScan.Z1

LSM 700

AxioScan.Z1

 

Objective

20×

20×

40×

20×

Channel 1

Reflector

DAPI

DAPI

DAPI

DAPI

Excitation wavelength

353

353

404

353

Emission wavelength

465

465

444

465

Channel2

Reflector

FITC

Cy3

Cy3

Cy3

Excitation wavelength

495

548

561

548

Emission wavelength

519

561

575

561

Channel 3

Reflector

 

FITC

FITC

FITC

Excitation wavelength

 

495

488

495

Emission wavelength

 

519

517

519

Channel 4

Reflector

   

Cy5

Excitation wavelength

   

650

Emission wavelength

   

673