Fig. 2
From: Biochemical and pathological changes result from mutated Caveolin-3 in muscle
The CAV3 p.P104L mutation causes muscle pathology and influences protein stability. (a) Macroscopic comparison of hind limbs at 26 weeks wildtype mouse and a CAV3 (p.P104L) transgenic mouse reveals a moderate decline in muscle mass in the transgenic mouse which more pronounced at 52 weeks. (b) H&E staining reveals a largely normal appearance of wildtype quadriceps muscle at age 26 weeks compared to the quadriceps muscle of a transgenic animal presenting with muscle fiber caliber variability and internalized muscle fiber nuclei (Paraffin sections) (c) Transcript studies of Cav3 in quadriceps muscle of 26 weeks old p.P104L CAV3 mutant animals and wildtype littermates revealed increased cDNA abundance p.P104L CAV3 mutants. (d) Immunoblot of quadriceps muscle protein extracts (8 and 26 weeks old animals) revealing a prominent statistically significant CAV3 decrease in the mutant muscle. Coomassie blue staining was used as loading control