Fig. 2

Satellite cell population is sortable from Pax7-YFP mice. a–c FACS profiles show a CD31⁻CD45⁻Sca1⁻Vcam1⁺ cell fraction of mononuclear cells isolated from limb muscles of wild-type mice (a). CD31⁻CD45⁻Sca1⁻Vcam1⁺ cells were sorted by FACS and stained with a PAX7 antibody (b) [quantified in (c)], scale bar 50 μm. Data represent the mean ± s.e.m. (n = 3). d A CD31⁻CD45⁻Sca1⁻Vcam1+ cell fraction was almost completely positive for YFP fluorescence. Top panel: FACS profiles of mononuclear cells derived from limb muscles of wild-type (Pax7^+/+) mice. Bottom panel: FACS profiles of mononuclear cells derived from limb muscles of Pax7^YFP/+ mice. e FACS plots demonstrated that YFP⁺ cells isolated from limb muscle of Pax7^YFP/+ mice were positive for Vcam1 and negative for CD31, CD45, and Sca1. YFP⁺ cells were stained with (lower panel) or without (upper panel) a Vcam1 antibody. f–j A YFP⁺ cell population was isolated from limb muscles of Pax7^YFP/+ mice by FACS without antibody staining. FACS profiles of mononuclear cells derived from Pax7^+/+ or Pax7^YFP/+ mice (f). The red circles indicate YFP⁺ cells. A representative image of isolated YFP⁺ cells cultured in growth medium (GM) for 3 days (g), scale bar 100 μm. YFP⁺ cells cultured in GM for 3 days as described in (g) were co-immunostained for YFP and PAX7 (h) or YFP and MyoD (i), scale bar 50 μm. Sorted YFP+ cells were quantified (j). Data represent the mean ± s.e.m. (n = 4)