Fig. 5

TrkC expression in situ and the percentage of TrkC-IR neuron innervation of muscle fibers. a NRG-1β: a1 NF-200-IR neurons, a2 TrkC-IR neurons, a3 SKM cells by α-actin, and a4 overlay of a1, a2, and a3. b NRG-1β + PD98059: b1 NF-200-IR neurons, b2 TrkC-IR neurons, b3 SKM cells by α-actin, and b4 overlay of b1, b2, and b3. c NRG-1β + LY294002: c1 NF-200-IR neurons, c2 TrkC-IR neurons, c3 SKM cells by α-actin, and c4 overlay of c1, c2, and c3. d NRG-1β + AG490: d1 NF-200-IR neurons, d2 TrkC-IR neurons, d3 SKM cells by α-actin, and d4 overlay of d1, d2, and d3. e control: e1 NF-200-IR neurons, e2 TrkC-IR neurons, e3 SKM cells by α-actin, and e4 overlay of e1, e2, and e3. Scale bar = 50 μm. 5-1 a–e TrkC-IR migrating neurons in different conditions. 5-2 a–e TrkC-IR neurons innervate muscle fibers. 5-1 f Quantification of the percentage of TrkC-IR migrating neurons. 5-2 f Quantification of the percentage of TrkC-IR neuron innervation of muscle fibers. Mean ± SD, n = 5. *P < 0.05, **P < 0.01, ***P < 0.001