Table 1 The primary and secondary antibodies for fluorescence labeling
Category | Antibodies | Concentration | Source |
|---|---|---|---|
Primary | Chicken polyclonal anti-MAP2 | 1:1000 | Abcam, Cambridge, MA |
Primary | Chicken polyclonal anti-NF-200 | 1:1000 | Abcam, Cambridge, MA |
Primary | Mouse polyclonal anti-α-actin | 1:50 | Abcam, Cambridge, MA |
Primary | Rabbit anti-TrkC polyclonal IgG | 1:500 | Abcam, Cambridge, MA |
Secondary | Goat anti-mouse IgG conjugated to DyLight 350 | 1:400 | ImmunoReagents, Chicago, IL |
Secondary | Goat anti-chicken IgG conjugated to DyLight 488 | 1:400 | ImmunoReagents, Chicago, IL |
Secondary | Goat anti-rabbit conjugated to Cy3 | 1:400 | Abbkine, Redlands, CA |
Secondary | Goat anti-mouse IgG conjugated to TRITC | 1:200 | ZSGB-BIO, Beijing, China |