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Fig. 4 | Skeletal Muscle

Fig. 4

From: Deletion of the microtubule-associated protein 6 (MAP6) results in skeletal muscle dysfunction

Fig. 4

Transversal microtubules organization differs between WT and MAP6 KO fibers. A FDB muscle fibers dissociated from WT and MAP6 KO adult mice were fixed at 37 °C before labeling with anti-β-tubulin (green) and anti-RyR1 (red) antibodies. Each image represents a single confocal plane. Scale bars: 10 μm. B The microtubule network density was assessed on 40 WT and 40 KO fibers depending on its orientation: either longitudinally oriented (a) or transversally oriented (b) compared to the fiber longitudinal axis. To get more details on microtubule network organization, longitudinal lines were drawn on each cell (c), and the intensity profile was recorded for each, allowing the determination of the number of peaks per micrometer for WT and MAP6 KO cells (d), which correspond to the number of transversal microtubules per micrometer. Values are represented as means ± SEM, Student’s t test, ***p < 0.001, **p < 0.01 and ns: non-significant

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