Fig. 5
From: Deletion of the microtubule-associated protein 6 (MAP6) results in skeletal muscle dysfunction
EM reveals a remodeling of the sarcoplasmic reticulum at the I-band in MAP6 KO fibers. a, b EM images of EDL fibers with transverse tubule (TT) staining (dark). In the insets, representative images of triads with TT pseudo-colored in green. Dotted circle in b points to an obliquely oriented TT, instead of the normal transversal TT orientation (thin black arrows). c, d EM pictures showing the organization of SR membranes at the I-band. Large arrows point to Z-lines. The free SR is colored in yellow and transverse tubules in green. e, f EM images of muscle cross sections. Empty arrows point to stacks of flat and parallel SR cisternae (one of them being enlarged in the inset). All images are representative of at least 30 fibers from 3 different animals of each genotype. Scale bars: a–b, 1 μm (inset: 0.05 μm); c–d, 0.2 μm; e–f, 0.1 μm (inset: 0.05 μm). g Representative western blots on two different mice of each genotype. h Quantitative analysis of CRC protein amounts: RyR1, alpha-1-subunit of DHPR, and SERCA in skeletal muscle homogenates. The amount of protein was normalized to GAPDH expression, the WT mean values being set to 1. n = 7 blots from three WT and three MAP6 KO mice, Mann-Whitney, ns: non-significant