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Fig. 3 | Skeletal Muscle

Fig. 3

From: Automated muscle histopathology analysis using CellProfiler

Fig. 3

Sample image processing by CellProfiler. a Original RBG image of mdx muscle section stained with laminin α2 (green) and DAPI (blue). b, c Converted grayscale images of each channel after running the “ColorToGray” module. d Inverted green channel image. e Pseudo-colored image to show individual muscle fibers identified by using ‘IdentifyPrimaryObjects’ module. f The identified muscle objects were shrunk by seven pixels in order to determine if they contain central nuclei. g Outlines of identified muscle fibers were overlaid with original green channel image to illustrate the accuracy of muscle fiber identification. h Pseudo-colored image to show individual nuclei identified by using ‘IdentifyPrimaryObjects’ module. i The outlines of identified muscle fibers and nuclei were overlaid with original green channel image to illustrate the accuracy of classification with either normal (blue) or central nucleated muscles (red)

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