Fig. 1

Dystrophin R16/17-syntrophin PDZ domain fusion protein restored sarcolemmal nNOSμ in mdx mice. a. Schematic outline of the fusion construct. R16/17, dystrophin spectrin-like repeats 16 and 17; Syn PDZ, syntrophin PDZ domain; Pal, the palmitoylation motif for membrane targeting. b. AAV viruses were injected into both TA muscles of six mdx mice (n = 6). Representative photomicrographs of serial muscle sections visualized for GFP, R17, syntrophin PDZ domain, and nNOS expression and nNOS activity from wild-type BL10 mice, untreated mdx mice, and AAV.R16/17-Syn PDZ.GFP.Pal treated mdx mice. Asterisk, the same myofiber in serial sections. Scale bar = 50 μm