Fig. 3

Deleting ZFP36L1 and ZFP36L2 in Pax7-expressing cells impairs skeletal muscle regeneration. The TA muscles of Zfp36L1/L2-P mice were injected with either CTX or PBS. Muscles were harvested at 1, 5, 10 or 25 days following treatment and transverse cross sections were generated. Controls represent Cre-negative littermates. a Sections were stained with haematoxylin (H; myofibre; pink) and eosin (E; nuclei; purple) to assess the skeletal muscle architecture. Open arrows identify centrally located nuclei, an indication of muscle regeneration. b Sections were Van Gieson’s stain to determine the extent of fibrosis. Closed arrows indicate fibrotic tissue (red). Insets of respective PBS-injected, uninjured contralateral TA muscles are in the left-hand corner of each image. Scale bars: 100 μm. Images representative of n = 3 at each time point