Fig. 4

Terminal differentiation can occur independently of ZFP36L1 and ZFP36L2. Transverse cross sections of injured TAs from Zfp36L1/L2-P mice were immunostained with antibodies for Pax7 (red), myogenin (myog; turquoise), laminin (lam; green), and counterstained with the nuclear marker DAPI (blue). Controls represent Cre-negative littermates. a Representative images of injured control and Zfp36L1/L2-P TAs. Insets represent PBS-injected, uninjured contralateral TA muscles. Open arrows indicate myogenin+ myogenic cells (myog; turquoise). Scale bars: 100 μm. b Average number of myogenin+ cells present in injured control and Zfp36L1/L2-P TAs during the injury time course. Images were acquired using a × 40 objective lens. Calculations were based on 10 fields of view per experiment. Error bars represent the SEM, two-way ANOVA adjusted for multiple comparisons with Sidak’s post hoc test, n = 3