Fig. 2
From: β-arrestin 1 regulates β2-adrenergic receptor-mediated skeletal muscle hypertrophy and contractility
β-arrestin 1 is required for enhanced Ca2+ transient in FDB fiber upon β2AR activation. Net peak amplitude of Ca2 + transient at different stimulation duration of FDB fibers after 2 weeks of stimulation with vehicle or clenbuterol (1 mg/kg/day) from WT (a) and βarr1KO (b). Data represent the mean ± SEM from at least five independent experiments. Statistical comparison of the curves was performed by using a two-way ANOVA with Sidak’s multiple comparison test (*, P < 0.05; ***, P < 0.001). Averaged peak fluorescence ratio ( ΔF/F0) (c) and basal transient (d) obtained from vehicle or clenbuterol (1 mg/kg/day) treated in WT and βarr1KO FDB fibers. Data are expressed as mean ± SEM from five independent experiments. Statistical comparison was performed by using a two-way ANOVA with Tukey’s multiple comparison test and student t-test (*, P < 0.05; **, p < 0.01; ***, P < 0.001)