Fig. 5

Human phospho-antibody array analysis reveals that phosphorylation events induced by clenbuterol in primary myoblasts are primarily β-arrestin 1–dependent. Normalized intensities for four representative phosphoproteins of ERK (a), CREB (b), GSK3 (c), and AMPK2 (d) applied to the phospho-antibody array. Lysates were from primary myoblasts isolated from WT and βarr1KO followed by 10 μM clenbuterol stimulation for 5 min or no stimulation (vehicle). The normalized intensity for each antibody was calculated as a fold increase over vehicle stimulation of primary myoblast from WT mice. Data shown represent the means ± SEM from five independent experiments. Statistical analysis was performed using a two-way ANOVA with Tukey’s multiple comparison test (*P < 0.05; **P < 0.01; ***P < 0.001)