Skip to main content

Advertisement

Fig. 2 | Skeletal Muscle

Fig. 2

From: Open-CSAM, a new tool for semi-automated analysis of myofiber cross-sectional area in regenerating adult skeletal muscle

Fig. 2

Open-CSAM comparison with MyoVision, MuscleJ, and SMASH softwares. The same pictures were analyzed either by manual measurement or using Open-CSAM (with or without manual correction), MyoVision, MuscleJ, or SMASH softwares. a Mean cross-section area (CSA) obtained on various Tibialis Anterior (TA) muscles. Muscles were isolated from 8- to 12-week-old mice uninjured (D0) or 8 days (D8), 14 days (D14), and 28 days (D28) post-cardiotoxin (CTX) injury, from 2-year-old mice uninjured (D0 old) or 28 days post-CTX injury (D28 old) and from dystrophic fibrotic mice (Fib-mdx). Results are mean ± SEM of 10 images from 2 muscles (Fib-mdx), 20 images from 2 muscles (D0 and D0 old), 30 images from 3 muscles (D28 and D28 old), 40 images from 4 muscles (D8), and 45 images from 4 muscles (D14). b Correlation between manual measurement (X axis) and Open-CSAM (without manual correction), MyoVision, MuscleJ, or SMASH (Y axis) measurements performed on the same images used in a. Each dot represents a picture. The dotted line represents the identity line. c Representative images measured manually, by Open-CSAM (before and after correction), MyoVision, MuscleJ, or SMASH softwares. Red fibers were false myofibers identified by the softwares, and green fibers were myofibers not considered by the Open-CSAM software and manually drawn. Lacking myofibers using MyoVision, MuscleJ, or SMASH softwares are shown by red asterisks. d Distribution of the CSA obtained with the six methods using the Fib-mdx samples used in a. Results are mean ± SEM of 10 images from 2 muscles (Fib-mdx). White bar = 25 μm. *p < 0.05, **p < 0.01, and ***p < 0.001 as compared with manual quantification

Back to article page