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Table 1 Recommended size and circularity thresholds

From: Open-CSAM, a new tool for semi-automated analysis of myofiber cross-sectional area in regenerating adult skeletal muscle

Muscle type Size threshold Circularity threshold
D0 (young and old) 200 μm2* 0.4
D8 50 μm2 then adjust* 0.4
D14 100 μm2 then adjust* 0.4
D28 (young and old) 150 μm2 then adjust* 0.4
Fib-mdx 50 μm2 0.4
  1. *If too many small myofibers are missed, progressively decrease the size threshold by testing on 2–3 representative pictures