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Table 1 Recommended size and circularity thresholds

From: Open-CSAM, a new tool for semi-automated analysis of myofiber cross-sectional area in regenerating adult skeletal muscle

Muscle type

Size threshold

Circularity threshold

D0 (young and old)

200 μm2*

0.4

D8

50 μm2 then adjust*

0.4

D14

100 μm2 then adjust*

0.4

D28 (young and old)

150 μm2 then adjust*

0.4

Fib-mdx

50 μm2

0.4

  1. *If too many small myofibers are missed, progressively decrease the size threshold by testing on 2–3 representative pictures
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Skeletal Muscle

ISSN: 2044-5040

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