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Table 2 Comparison of software characteristics

From: Open-CSAM, a new tool for semi-automated analysis of myofiber cross-sectional area in regenerating adult skeletal muscle

Program

Software base

OS

Minimal configuration tested

Manual correction

PC

MAC

Open-CSAM

ImageJ plugin

Mac OS/Windows

Windows 10-Intel core i3 CPU 550 3.2 GHz-4 Go RAM

Mac OS 10.12/Intel core i3–3.2 GHz/12 Go RAM

Yes—during the analysis

➔ Draw/delete ImageJ tools

MyoVision

Windows application

Windows

Windows 10-Intel core i3 CPU 550 3.2 GHz-4 Go RAM (single image analysis)/Windows 7-Intel core i5 vpro 3.1 GHz/16 Go RAM (whole muscle analysis)

Not applicable

No

MuscleJ

ImageJ plugin

Mac OS/Windows

Windows 10-Intel core i3 CPU 550 3.2 GHz-4 Go RAM

Mac OS 10.13/Intel core i5–2.7 GHz/16 Go RAM

Yes—after the analysis

➔ Open picture

➔ Import ROI file on the picture

➔ Draw/delete ImageJ tools

SMASH Stand Alone

Windows application

Windows

Windows 10-Intel core i3 CPU 550 3.2 GHz-4 Go RAM

Not applicable

Yes—during the analysis

➔ Step 1 draw—validate

➔ Step 2 delete—validate

(cumbersome: small screen, no back and forth allowed between steps 1 and 2)