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Fig. 5 | Skeletal Muscle

Fig. 5

From: Normal inflammation and regeneration of muscle following injury require osteopontin from both muscle and non-muscle cells

Fig. 5

Inflammatory cells in autografted and allografted extensor digitorum longus muscles. a Representative transverse sections of EDL muscle grafts 1 day after grafting, immunostained with anti-Ly6G (green) and counterstained with DAPI (blue). Four combinations of wild-type (WT) and osteopontin-null (KO) host and graft. Arrows indicate neutrophils. Bar = 50 μm. b Quantitation of neutrophils in Ly6G-stained sections of grafts (expressed as neutrophils/field). ce Quantitation of macrophages by flow cytometry. Numbers of total (F4/80-positive; c), M1 (d) and M2 (e) macrophages are expressed as cells/muscle, and M1 and M2 macrophage numbers are also expressed as a percentage of total macrophages (d, e). Data are presented as mean ± s.e.m. in n = 6 animals. Data were analysed using one-way ANOVA with Tukey’s multiple comparison test. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 for comparisons between graft types indicated by lines on graph. fh Representative FACS scans showing gating of monocytes based on forward and side scatter (f), F4/80 staining (g) and CD206 and CD11c staining (h)

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