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Table 1 Antibodies used for immunohistochemistry

From: Normal inflammation and regeneration of muscle following injury require osteopontin from both muscle and non-muscle cells

Primary antibody Secondary antibody
FITC-conjugated rat anti-mouse Ly6G, Clone 1A8 (1:200; BD Biosciences, San Jose, CA, USA) Not applicable
Rat anti-mouse F4/80 IgG2b Clone Cl:A3-1 (1:500; AbD Serotec, Oxford, UK) Alexa Fluor 488-conjugated goat anti-rat IgG AB_2534074 (1:100; Abcam, Cambridge, MA, USA)
Goat anti-mouse osteopontin IgG ab11503 (10 μg/ml; R&D Systems, Minneapolis, MN, USA) Alexa Fluor 594-conjugated donkey anti-goat IgG AB_2534105 (1:200; Abcam)
Mouse embryonic myosin heavy chain (F1.652.5; 2 μg/ml; Developmental Studies Hybridoma Bank, University of Iowa) Alexa Fluor 488-conjugated goat anti-mouse IgG AB_2534069 (1:100; Life Technologies)
Mouse anti-desmin Clone DE-U-10 (1:80; Sigma-Aldrich St. Louis, MO, USA) Alexa Fluor 488-conjugated goat anti-mouse IgG AB_2534069 (1:100; Life Technologies)
Rabbit anti-laminin L9393 (1:100; Sigma-Aldrich) TRITC-conjugated swine anti-rabbit IgG A-11029 (1:100; Dako Cytomation, Glostrup, Denmark)