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Fig. 3 | Skeletal Muscle

Fig. 3

From: Late-onset megaconial myopathy in mice lacking group I Paks

Fig. 3

Myopathic dKO muscles are not obviously dystrophic. a Analysis of Pax7 and Ki67 expression by satellite cells in TA muscles of 1-year-old control and dKO mice. Pax7+ cells are indicated by arrows; Pax7+/Ki67+ cells are indicated by arrowheads. Individual myofibers are revealed by labeling with wheat germ agglutinin (WGA; green), and nuclei are identified by DAPI (blue). The top panels of the insets are the cells identified by arrowheads, and the bottom panels of the insets are the cells identified by arrows. The histograms quantify the average number of Pax7+/DAPI+ cells per mm2 (left) and the average number of Pax7+ cells that are also Ki67+ (right). n = 3 mice per genotype, with each point representing the average value from 20 randomly selected sections per mouse. Values are means ± SD, *p < 0.05. b TA muscles of 1-year-old control and dKO mice immunostained for dystrophin (magenta) and DAPI (blue). c Comparative evaluation of myofiber membrane permeability by Evans blue dye (EBD; magenta) uptake in 1-year-old animals. The experiments in b and c were repeated three times, with similar results

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