Fig. 6

Analysis of choline kinase expression in muscle and C2C12 myoblasts. a Western blot analysis of choline kinase (Chk) isoform expression in quadriceps extracts from 1-year-old control and dKO mice. Brain protein lysate used as a positive control for Chk antibodies. Two mice of each genotype are shown. b Densitometric quantification of the results in a and similar western blots; Gapdh was used for normalization. n = 5 mice per genotype, *p < 0.05. c Western blot analysis of the indicated proteins in C2C12 cells cultured in growth (G) medium and for the indicated number of days in differentiation (D) medium. d Densitometric quantification of the results in c and similar western blots; Gapdh was used for normalization. n = 3 independent experiments, one-way ANOVA followed by Dunnett’s multiple comparison tests was used for statistical analysis. The means of all groups were compared to growth conditions, *p < 0.05