Fig. 2

The effect of IMB0901 in the in vitro model. a The effect of IMB0901 on the survival rate of C2C12 cells. MTT assay was used to detect the survival rate of C2C12 cells after 24-h and 48-h treatment with IMB0901 at various concentrations. The values presented means ± SD from three experiments, each performed in triplicate. b The inhibition of IMB0901 on the MSTN mRNA expression. Real-time PCR was used to detect the effect of IMB0901 on the upregulation of MSTN during C2C12 myotube atrophy induced by C26 cell supernatant at mRNA level. There were four groups in this experiment: control (no CM or IMB0901), CM (1/6 CM), IMB0901 (1/6 CM + 2.5 μM IMB0901), and IMB0901 (1/6 CM + 5 μM IMB0901), for the following data. The data presented means ± SD from three experiments, each performed in triplicate. **P < 0.01 compared to the control group, ^#P < 0.05 compared to the CM group. c The inhibition of IMB0901 on the MSTN protein expression. Western blotting was used to detect the effect of IMB0901 on the upregulation of MSTN during C2C12 myotube atrophy induced by C26 cell supernatant at protein level. The data presented means ± SD from three experiments. **P < 0.01 compared to the control group, ^##P < 0.01 compared to the CM group. d The inhibition of IMB0901 on the MSTN signaling pathway in the in vitro cell model. Western blotting was applied to assess the effect of IMB0901 on the phosphorylation level of SMAD and FOXO3a during the process of C2C12 myotube atrophy induced by C26 cell supernatant. The values presented means ± SD from three experiments. **P < 0.01 compared to the control group, ^#P < 0.05 and ^##P < 0.01 compared to the CM group