Fig. 4

The mechanism of IMB0901 in the in vitro model. a The inhibitory effect of IMB0901 on two E3 ligases, Atrogin-1 and MuRF-1. Western blotting was used to detect the effect of IMB0901 on the expressions of two E3 ligases, Atrogin-1 and MuRF-1, during the process of C2C12 myotube atrophy induced by C26 cell supernatant. b The inhibitory effect of IMB0901 on the ubiquitin expression. Western blotting was used to detect the effect of IMB0901 on the ubiquitin expressions during the process of C2C12 myotube atrophy induced by C26 cell supernatant. c, d, e The positive effect of IMB0901 on phosphorylation level of AKT, P70S6K and mTOR in atrophied C2C12 myotubes. Western blotting was used to detect the phosphorylation of AKT, P70S6K and mTOR during the process of C2C12 myotube atrophy induced by C26 cell supernatant. The values were means ± SD from three independent experiments. *P < 0.05 and **P < 0.01 compared to the control group, ^#P < 0.05 and ^##P < 0.01 compared to the CM group