Fig. 3From: Exogenous expression of the glycosyltransferase LARGE1 restores α-dystroglycan matriglycan and laminin binding in rhabdomyosarcomaDystroglycan glycosylation in primary cell cultures derived from human rhabdomyosarcomas. a–c. Western blotting of WGA-enriched protein samples extracted from human aRMS (PCB82 and PCB232), eRMS (PCB380), and C2C12 cells for core α-dystroglycan (a), matriglycan-modified α-dystroglycan (b), and laminin-bound dystroglycan (c). d–f Relative expression of mRNAs encoding dystroglycan (DAG1) and the dystroglycan-modifying glycosyltransferases in two aRMS and one eRMS line vs. skeletal muscle, as quantified by real-time PCR. * These values are statistically different according to unpaired two-sample t test, p < 0.05Back to article page