Fig. 1

ENT treatment of eRMS in vivo. a Box-and-whisker plots showing the tumoristatic efficacy of entinostat in combination with vincristine in eRMS mice (DMSO vs Ent + VCR. Data are presented as means ± SEM (N = 5 mice per cohort). ***P < 0.001 by log-rank test. b Kaplan-Meier plots of eRMS mice treated with ENT at a daily dose of 5 mg/kg administered intraperitoneally, VCR at 1 mg/kg administered intraperitoneally once a week or a combination of both. Treatment was stopped early for all mice after day 11 because of body weight loss in some of the animals. Data are presented as means ± SEM (N = 5 mice per cohort). ***P < 0.001 by log-rank test. c Samples were collected from mice upon necropsy at study completion. Tumors were stained by hematoxylin and eosin and scored blindly. Rhabdomyoblasts are visible in ENT + VCR treated cells (pointed with white arrowhead). Scale bar, 100 μM