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Fig. 2 | Skeletal Muscle

Fig. 2

From: Automated image-analysis method for the quantification of fiber morphometry and fiber type population in human skeletal muscle

Fig. 2

Example of automated analysis results. Human deltoid muscle cross-section analyzed with our macro in Fiji-ImageJ. a Cross-sections are labeled for dystrophin and spectrin (green—channel 1) to outline the fibers and for myosin heavy chain (MyHC) (red—channel 2) to identify type II myofibers; type I myofibers are unlabeled. Images were acquired with a Zeiss epifluorescence microscope using a × 10 objective and saved in a 16-bit TIF format. Original pictures of individual channels were used for the analysis. b After image enhancement and cleaning steps, channel 1 is used to segment and outline the membrane of all fibers; a flattened image is automatically saved. c Channel 2 is then used to identify labeled cells within those previously identified cells. d Non-labeled fibers are then calculated by difference, and type I myofibers are segmented. To facilitate the visual identification of fiber’s size distribution, color-coded maps were obtained based on the myofibers. e Cross-sectional area (from ≤ 1000 to > 4000 μm2). f Minor diameter (from ≤ 10 to > 70 μm). Scale bar represents 500 μm

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