Fig. 6
Example of color-coded images as a tool to visually detect changes in myofibers’ size. Color-coded maps were obtained based on the myofibers’ cross-sectional area (from ≤ 1000 to > 4000 μm2). Scale bar represents 500 μm. Representative images from a lean (male 43 yo, BMI 21.7), b obese (male 43 yo, BMI 33.2), c young (male 34 yo, BMI 21.71), and d old (male 77 yo, BMI 22.5) subjects from the healthy patient cohort. Characteristics of these patients are described in Table 3. Left: original immunofluorescence-stained section. Right: color-coded image. MyHC, myosin heavy chain; yo, years old; BMI, body mass index