Fig. 4

Genetic ablation of cIAP2 does not attenuate denervation-induced atrophy. A small (5–10 mm) piece of the right sciatic nerve was removed to induce denervation of the entire right hind limb of 6-week-old C57BL/6 mice (blue bars) or sex- and age-matched cIAP2-null mice (yellow bars). The contralateral leg served as an internal control. Mice were sacrificed 14 days post-denervation. a Representative Western blots of cIAP1 and cIAP2 protein expression of the heart and spleen of C57BL/6, cIAP1-null, and cIAP2-null mice. b Representative images of TA muscle sections stained with H&E from mice described above. Scale bar = 20 um. c Loss in TA fiber size as a percentage of non-denervated cross-sectional area (n = 3). d Representative Western blots of cIAP1, MuRF1, and GAPDH protein expression in gastrocnemius muscle from non-denervated (NDN) and denervated (DEN) hind limbs of C57BL/6 and cIAP2-null mice. Data is the mean ± SEM, n.s. not significant