Fig. 3
From: Culturing C2C12 myotubes on micromolded gelatin hydrogels accelerates myotube maturation
Contractile protein content increases in C2C12 myotubes grown on micropatterned gelatin hydrogels compared to other culturing methods. a. Representative SDS-PAGE gel separating total protein content of C2C12 myotubes at days 4 and 7 post-differentiation on plastic cell culture dishes, unpatterned gelatin hydrogels (UP), and micropatterned gelatin hydrogels. b Myosin heavy chain content normalized to total protein loaded. c Actin compared to total protein loaded. d Myosin heavy chain to actin ratio. Sample size per group, n = 3 biological replicates. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001