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Fig. 4 | Skeletal Muscle

Fig. 4

From: Culturing C2C12 myotubes on micromolded gelatin hydrogels accelerates myotube maturation

Fig. 4

Global transcriptomic characterization of patterned and unpatterned myotubes by RNAseq. a Schematic describing samples used for RNAseq. GO categories enriched in all comparisons of patterned vs. unpatterned or plastic myotubes. Genes in the sarcomere category (highlighted in blue) were used for downstream analysis. b Cumulative distribution of the log2 fold change (LFC) of all genes (black) and sarcomere genes (blue), where LFC is computed using batch 1, day 7 patterned vs. unpatterned myotubes. Inset: bar showing the difference between median sarcomere LFC and median LFC for all genes, defined as sLFC (sarcomere log fold change). c Bars showing sLFC for each comparison of patterned vs. unpatterned or plastic. d In vivo time course used to select P2 vs. E18 upregulated genes [40]. Cumulative distribution of the LFC of all genes (black) and in vivo upregulated genes (orange), where LFC is computed using batch 1, day 7 patterned vs. unpatterned myotubes. Inset: bar showing the difference between median in vivo upregulated LFC and median LFC for all genes, defined as vLFC (in vivo log fold change). e Bars showing vLFC for each comparison of patterned vs. unpatterned or plastic. f Cumulative distribution of LFC for all genes (black) and in vivo upregulated genes (orange), where LFC is computed using primary myoblasts at day 7 vs. day 0. g Points showing vLFC for all day 7 vs. day 0 myoblast comparisons (blue) and primary myoblasts (orange). The statistical significance of the primary myotube versus C2C12 myotubes was assessed by computing a Z score and associated P value from the mean and standard deviation of the C2C12 myotubes

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