Fig. 2

Estrogen signaling interferes with muscle inflammation and regeneration after acute myoinjury. a Elisa assay showing serum E₂ levels in CTX-treated mice received β-estradiol or 4-OHT administration (on day 1 post-injection), or in ovariectomy (OVX) mice. b Histological features of damaged TA muscle in CTX-treated mice received β-estradiol or 4-OHT administration, or in CTX-treated OVX mice. c Immunofluorescence staining results of CD11b, F4/80 and Dystrophin in damaged TA muscle from B6 mice received β-estradiol or 4-OHT administration, or performed OVX. d FACS analysis of the proportion of CD11b⁺ and F4/80⁺ cells in CD45-gated cells isolated from TA muscle on day 3 post-injury in OVX or intact mice. Data are representative of two independent experiments performed with three mice per group. One-way ANOVA was used for multiple comparisons. (*p < 0.05). Bar = 100 μm