Fig. 4

Estrogen signaling affects on accumulation and function of CD4⁺ cells in damaged muscle. a Immunofluorescence double-staining (left) and FACS analysis (right) of CD4⁺ T cells in inflamed muscle. b FACS analysis of the frequency of Th1(IFN-γ⁺), Th2(IL-4⁺), and Th17(IL-17⁺) cells gated from CD4⁺ cells isolated from 7d-damaged TA muscle, in OVX or intact female mice. c mRNA levels of cytokine IFN-γ, IL-4, IL-17 and transcription factor T-bet, IRF4, STAT3 in CD3⁺CD4⁺ T cells isolated from 6d-damaged TA muscle in OVX or intact female mice, were quantified by qRT-PCR. Data are representative of two independent experiments performed with three mice per group. One-way ANOVA was used for multiple comparisons (*p < 0.05). Bar = 50 μm