Fig. 2

Effects of ibuprofen on cell proliferation and apoptosis. Skeletal muscle cells were treated with 0.05 mg/ml, 0.1 mg/ml, 0.2 mg/ml, and 0.4 mg/ml ibuprofen for 24 h and cell viability was determined by the MTT assay (a). The TUNEL-positive nuclei were stained with FITC (green), and all nuclei were stained with DAPI (blue). The micrographs were obtained at × 200 magnification. The cells treated with DNase I were used as positive controls (b). Data represented the mean ± SEM of three independent experiments. *Mean p < 0.05 compared to the control. Scale bars, 50 μm