Fig. 4From: Ibuprofen inhibited migration of skeletal muscle cells in association with downregulation of p130cas and CrkII expressionsIbuprofen reduced cell spreading of skeletal muscle cells. Skeletal muscle cells were treated with 0.05 mg/ml, 0.1 mg/ml, 0.2 mg/ml, and 0.4 mg/ml ibuprofen for 24 h. After plating for 30 min, the attached skeletal muscle cells started to spread out and were observed via F-actin staining (a). The spread cells were indicated by the white arrows. F-actin was stained green, and nuclei were stained blue. The percentage of spreading cells out of adhered cells was shown in b, and the data represented the mean ± SEM% of three independent experiments. *Mean p < 0.05 compared to the control. Scale bars, 100 μmBack to article page