Fig. 1
From: Defective angiogenesis in CXCL12 mutant mice impairs skeletal muscle regeneration
CXCL12Gagtm/Gagtm mice show no systemic developmental or morphological defects but signs of vascular destabilization. a, b Representative pictures of HE-stained sagittal cross section of 8-week-old a WT (C57Bl6) mice and b KI (CXCL12Gagtm/Gagtm) mice. Scale bar represent 10 mm. Muscle morphology comparison between the KI and the WT mice on c, d HE stained tibialis anterior (TA) sections from c WT and d KI mice. Scale bar represent 100 μm. e, f Sirius Red (collagen deposits) staining in TA sections from e WT and f KI mice. Scale bar represent 50 μm. g, h Endothelium (CD31, red) and muscular basal lamina (laminin, green) immunolabeling counterstained for nuclei (DAPI, blue) in TA sections from g WT and h KI mice. Scale bar represent 50 μm. i, j Longitudinal blood vessel organization of sectioned TA from i WT (Flk1GFP/+) and j KI (CXCL12Gagtm/Gagtm:Flk1GFP/+) mice. White arrowheads point towards the sprouting structures. Scale bar represent 10 μm. k, l SC (Pax7-GFP, green) location, basal lamina (laminin, blue), and vessels (CD31, red) immunostained in TA sections from k WT (Tg:Pax7nGFP) and l KI (CXCL12Gagtm/Gagtm:Pax7nGFP) mice. Scale bar represent 10 μm (n = 3 animals per condition). All the experiments were repeated independently two times