Fig. 2

Transcriptome analysis of the SCs and the ECs from the uninjured TA of the KI versus the WT mice and in vivo angiogenesis assay. a Venn diagram of unique and overlapping differentially represented (q value < 0.1) Gene Ontology Biological Pathways (GOBP) in KI versus WT mice depending on the cell type (Pax7/GFP-positive and Flk1/GFP-positive cells). b Simplification of GOBP terms into GOBP slim terms, along with the number of differentially represented GOBP terms participating in each GOBP slim. c Transcriptional profile of the 30 most significantly differentially expressed genes in the selected GOBP. Expression of genes is presented as centered and scaled log2 fluorescence intensity (red to yellow key), and each row represents a gene, named by its MGI symbol (n = 3 animals were used per condition). d, e Representative immunostaining of Matrigel plugs mixed with KI (CXCL12^Gagtm/Gagtm:Pax7nGFP) satellite cells inserted for 3 weeks in either d WT (C57Bl6) or e KI (CXCL12^Gagtm/Gagtm) mice. Endothelial cells (CD31, red), myoblasts (desmin, green), and nuclei (DAPI, blue) were immunolabeling. Scale bars represent 20 μm. f Quantification of the CD31 positive/negative surface ratio as an indicator of vessel formation. Data are mean percentage ± SEM (10 fields per Matrigel plug). n = 5 animals per condition. All the experiments were repeated independently two times. *p < 0.05