Fig. 8

LMCD1 regulates myoregulin (Mln) expression and depends on calcineurin signaling. a qRT-PCR of Mln in gastrocnemius after 7 days of GFP (control) or LMCD1 expression (n = 6). b qRT-PCR Mln in gastrocnemius after 7 days of shControl or shLmcd1 delivery to muscle (n = 6). c Luciferase activity of control vector or MLN promoter (with no mutations, mutations on the myocyte enhancer factor-2 (MEF2) domain, on the enhancer box (EBox) domain or in both domains) in C2C12 cells transfected with scrambled siRNA or siCalcineurin (siCnA) and 2 days after transduction with GFP (control) or LMCD1 adenovirus (n = 5). Data is shown as mean ± SEM and *p < 0.05; **p < 0.01