Fig. 2

Impaired mitochondrial fatty acid oxidation in H6PDKO skeletal muscle. a High resolution respirometry of fatty acid oxidation in permeabilised tibialis anterior WT (n = 3) in and H6PDKO (n = 3). b High-resolution respirometry of fatty acid oxidation using WT (n = 3) and H6PDKO (n = 3) permeabilised. c Mitochondrial DNA (mtDNA) quantification of WT (n = 7) and H6PDKO (n = 7) muscle, measured using qRT-PCR. d Western blots of WT and H6PDKO protein lysates (n = 9) probed for oxidative phosphorylation enzyme subunit abundance. e Acylcarnitine species levels in WT (n = 9) and H6PDKO (n = 11) muscle measured using GC-MS/MS. Data expressed as heat-maps with log2 values representing metabolite abundance in WT and H6PDKO. Box and Whisker plots showing significantly altered short acylcarnitines. f qRT-PCR measurement of genes critical carnitine and fatty acids metabolism in WT (n = 7) and H6PDKO (n = 7) TA. g Schematic showing carnitine and fatty acid metabolism between cytosol and mitochondria. h Western blots of acetylated proteins within WT (n = 6) and H6PDKO (n = 6) skeletal muscle. *P < 0.05, **P < 0.01 and ***P < 0.001