Fig. 2

Depletion of rictor does not affect muscle structure and force. a Muscle mass, normalized to body mass, of tibialis anterior (TA), extensor digitorum longus (EDL), soleus (Sol), and gastrocnemius (Gastro) muscles from 5-month-old male RImyfKO mice and control (Ctrl) littermates (n = 4-6). b Hematoxylin and eosin (H&E) coloration of TA cross-sections of 5- and 14-month-old Ctrl and RImyfKO mice. No alteration in muscle histology and fiber organization was detected in RImyfKO muscle. Scale bar, 50 μm. c Immunostaining against laminin (red) and myosin heavy chain IIa + x (green) of EDL cross-sections from 14-month-old Ctrl and RImyfKO mice. Type IIa fibers are labeled in bright green, type IIx in light green and IIb fibers are unstained. Scale bar, 100 μm. d–f Fiber size distribution (d), mean of minimum feret diameter (e), and total number of fibers (f) were analyzed in EDL muscle of 14-month-old Ctrl and RImyfKO mice (n = 3). g Specific twitch force (sPt) and specific tetanic force (sP0) of EDL and Sol muscles from 14-month-old Ctrl and RImyfKO mice (n = 4–7). Data represent mean ± SEM. Student’s t test