Skip to main content
Fig. 5 | Skeletal Muscle

Fig. 5

From: mTORC2 affects the maintenance of the muscle stem cell pool

Fig. 5

mTORC2 signaling does not affect MuSC quiescence and differentiation. ad Single EDL myofibers were isolated from 5-month- (ac) or 14-month-old (d) control and RImyfKO mice and cultured for different time points. a Immunostaining against Pax7 (red) and DAPI staining (blue). Scale bar, 20 μm. b Relative number of Pax7- and MyoD-positive cells at time point zero (t0), after 24 h (t24), 48 h (t48), and 72 h (t72) in culture. c Mean number of myogenic cells per myofiber at the different time points, normalized to t0. d Relative number of Pax7- and MyoD-positive cells at t72 in myofibers isolated from 14-month-old mice. Data represent mean ± SEM (n = 3–4 at t0, 3 at t24, 10–11 at t48, and 7 at t72; 20–40 myofibers per animal). Statistics used Student’s t test

Back to article page