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Fig. 1 | Skeletal Muscle

Fig. 1

From: Development of a high-throughput screen to identify small molecule enhancers of sarcospan for the treatment of Duchenne muscular dystrophy

Fig. 1

Individual components of muscle adhesion complexes increase during C2C12 differentiation. Confluent C2C12 myoblasts (day 0, D0) were switched from proliferation to differentiation media and harvested daily for 7 days (D1 to D7). Individual genes encoding protein components of the three major adhesion complexes (DGC, UGC, and α7β1D-integrin complex) were investigated, including a SSPN, sarcospan; b DMD, dystrophin; c UTRN, utrophin; d DAG, dystroglycan; e SCGA, α-sarcoglycan; f SCGB, β-sarcoglycan; g ITGA7, α7 integrin; and h ITGB1, β1D integrin. Analysis of myogenin (MYOG, i) and myogenic factor 5 (MYF5, j) are provided as markers for muscle cell differentiation. For DMD D0, n.d. (no data) indicates no detectable expression. Gene expression was calculated using the ddCt method and normalized to β-actin with day 0 (myoblast) values serving as the calibrator sample (n = 3). R.U., relative units. Results are plotted on a log scale. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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