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Fig. 5 | Skeletal Muscle

Fig. 5

From: Expressing a Z-disk nebulin fragment in nebulin-deficient mouse muscle: effects on muscle structure and function

Fig. 5

Z-disk width and thin filament length analysis. a Z-disk width distribution in CTRL (left) and Neb cKO (right) TC muscle. In CTRL mice, the Gaussian curve fit is significantly right-shifted in AAV-treated muscles by ~ 17 nm (p < 0.0001). (Note that while vehicle-treated CTRL mice have a double peak, it was not sufficient to qualify this data for a sum of two Gaussians curve fits.) In Neb cKO mice, the Gaussian curve fit is significantly left-shifted by ~ 6 nm (p < 0.0001). Measurements were made using electron microscopy images. (Analysis restricted to sarcomeres devoid of rod bodies. 8–17 fibers from 2 CTRL and 2 Neb cKO mice, with an equal number of Z-disks sampled from each fiber.) b Average Z-disk widths. Treatment with the Z-disk AAV causes a significant widening of the Z-disks in CTRL mice and a slight, but significant, decrease in the Neb cKO mice. c Thin filament lengths. No effect of AAV treatment on thin filament length in either genotype. (Measurements based on phalloidin-labeled sections imaged by deconvolution microscopy. In these experiments, a 1× AAV dosage was used. SL: 2.57 ± 0.05 μm (n = 45; CTRL, VEH), 2.57 ± 0.04 μm (n = 33; CTRL, AAV); 2.18 ± 0.16 μm (n = 41; Neb cKO, VEH); 2.18 ± 0.13 μm (n = 79; Neb cKO, AAV). (Unpaired t tests with Welch’s correction were performed)

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