Fig. 8
Fatigability of muscles treated with a 1× and 3× dose of nebulin’s Z-disk region. a Fatigue protocol consisting of 75 stimulations (1-s 60 Hz stimulation, 2-s rest) performed in 1× (left) and 3× (right) dosage groups. Notably, Neb cKO mice are more fatigue resistant due to previously reported changes in fiber-type composition [36]. b Quantification of force produced at the final stimulation of the 1× (left) and 3× (right) dosage group fatigue protocols. In both dosage groups, Neb cKO mice are found to produce more force at the end of the protocol (paired t test). c Force produced during the final fatigue stimulation as a percentage of the average of the force produced in the first five stimulations in 1× (left) and 3× (right) dosage groups. Higher percentage indicates resistance to fatigue. (1×: n = 9, 11 mice; 3×: n = 4, 9 mice)