Fig. 2
Transduction of myotubes with HERG1A-encoded adenovirus is a valid in vitro skeletal muscle atrophy model. a The area of myotubes treated with HERG1A-encoded adenovirus is a significant 26.4% smaller (p < 0.01; n = 3 experimental sets) than that of control myotubes at 48 h after transduction and a significant 19.3% smaller (p < 0.01; n = 3 experimental sets) at 72 h after transduction. Scale bar = 100 μm. Bars of the graph represent the mean myotube area (μm2) while the error bars represent the standard error of the mean. b Immunoblot shows that transduction of C2C12 myotubes with a HERG1A-encoded adenovirus yields an early increase in MuRF1 E3 ligase protein abundance while it does not increase abundance of ATROGIN1 protein. Immunoblots are representative of three experiments