Fig. 3
Transduction of myotubes with HERG1A-encoded adenovirus increases basal intracellular calcium levels and basal calpain activity. a Fura-2 dye experiments reveal that expression of HERG1A in C2C12 myotubes yields a 51.9% increase (p < 0.0001; n = 90 GFP and n = 87 HERG1A transduced wells) in basal intracellular calcium levels relative to myotubes transduced with a control virus. b Calpain assay reveals that transduction of C2C12 myotubes with a HERG1A-encoded adenovirus increases combined native calpain 1 and 2 activity a significant 31.9% (p < 0.08; n = 24; two-way ANOVA) over control myotubes. All bars represent the mean while error bars represent the standard error of the mean