Fig. 1

Transplantable model for lung cancer-associated tissue loss. a Schematic of the study design. D0 = day tumor cells were injected into mice. All studies were survival studies; therefore, endpoint was variable. The median survival was 26 days. echoMRI scans were completed twice per week during the course of the study. b Survival curve for vehicle and tumor injected cells. Dotted vertical line indicates the median survival (26 days). n = 10 vehicle, 15 tumor. Data were compiled from 3 independent cohorts of animals. Survival is statistically different (p < 0.0001) by Gehan-Breslow-Wilcoxon test. c Representative images of lungs from vehicle and tumor-bearing mice. Ruler reference in centimeters. d Representative hematoxylin and eosin (H&E) staining of lung tissue from vehicle (left) and tumor-bearing (right) mice. Two representative images from 2 individual animals in each condition. Scale bar is 800 μm. e Total lung weight (all lobes) from vehicle and tumor-bearing mice. Individual points represent individual animals. Boxes represent the inner quartiles, and whiskers represent the minimum and maximum values. f Total mouse weight across the study, normalized to pre-tumor baseline weight. Error bars are SEM. p values presented in the figure are the result of mixed-effects analysis, with Geisser-Greenhouse correction. Significance at individual points was determined by correction for multiple testing using false discovery rate (Benjamini and Hochberg). g echoMRI calculated total fat mass across study, normalized to pre-tumor baseline fat mass. Error bars are SEM, and statistical analysis is as described above. h echoMRI calculated total lean mass across study, normalized to pre-tumor baseline lean mass. Error bars are SEM, and statistical analysis is as described above. *p < 0.05 by Student’s t test n = 5 vehicle, 4 tumor-bearing 7-week-old male 129S2/SvPasCrl mice for all data except survival curve